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Some engineering continues to be done to Enhance the in vitro exercise of T4 DNA ligase; one particular successful method, as an example, analyzed T4 DNA ligase fused to numerous option DNA binding proteins and found that the constructs with both p50 or NF-kB as fusion companions were above one hundred sixty% a lot more Energetic in blunt-stop ligations for cloning functions than wild style T4 DNA ligase.[6] A normal reaction for inserting a fragment right into a plasmid vector would use about 0.01 (sticky ends) to 1 (blunt ends) models of ligase. The optimum incubation temperature for T4 website DNA ligase is sixteen °C.[citation wanted]
Although DNA self-assembly is achievable without any outdoors help employing various substrates such as provision of catatonic floor of Aluminium foil, DNA ligase can offer the enzymatic assistance that is necessary to produce DNA lattice structure from DNA more than hangs.[15] History[edit]
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DNA ligases are getting to be indispensable applications in fashionable molecular biology exploration for creating recombinant DNA sequences. For example, DNA ligases are made use of with restriction enzymes to insert DNA fragments, normally genes, into plasmids.
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